On 24/10/2011 3:54 AM, DK wrote:
In article<[email protected]>,
lautys<[email protected]> wrote:
APS should prepare fresh every time you do your gel.
For how much longer will this myth be propagated?
There is nothing wrong with aliquoting APS and storing it at -20. I've used APS
solutions that were certain to be close to a year old stored at -20C and it
still
worked fine with no obvious change in polymerization time (0.05-0.1% APS
final).
DK
I was going to jump in with a similar comment but you beat me to it!
I also aliquot my APS and store at -20C with no loss in activity. It's
also ok for a several days at 4C (I used it up to a week later). Sure
beats having to weigh it out each time.
Allison
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