Sudheer, If you need help, you need to provide information on what you did. You cannot ask people to read up articles for you. Please provide the protocol briefly. If a gel does not polymerise, it is usally something to do with the proton donor used and the catalyst, whcih are usually APS and TEMED which helps Bis to crosslink the acrylamide monomers. The second reason might be badly oxidised or old acrylamide or Bis-acrylamide or their solutions, third reason might be (very unlikely) dirty plates which were not washed properly. There could be a host of other reasons and variables. So provide a detailed protocol. DO NOT attach it. Bionet does not allow attachments or website addresses. Jay
________________________________________ From: [email protected] [[email protected]] On Behalf Of sudheer sangeetham [[email protected]] Sent: Thursday, October 20, 2011 9:14 AM To: [email protected] Subject: Hello...Native Page is not polymerising Hello Could any please tell me why my Native PAGE is not polymerizing. I have referred one article, which i mentioned below and did in similar way and same concentrations but didnt polymerized. Could any one please tell me the reason. Thank you very much in advance Best Regards -- Sudheer Babu.S Ph.D student Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
