I also concur about the Aps most likely being inactive. How do you store it? I suggest a vacuum dessicator.
If the acryl amide or bis was bad, you would see increased viscosity if you buy it dissolved. Keep it in the dark, as light can initiate polymerization. On Oct 22, 2011 4:35 PM, "Dr Engelbert Buxbaum" < [email protected]> wrote: > In article <[email protected]>, > [email protected] says... > > > > Hello > > Could any please tell me why my Native PAGE is not polymerizing. I have > > referred one article, which i mentioned below and did in similar way and > > same concentrations but didnt polymerized. Could any one please tell me > the > > reason. > > Since you did not provide the actual paper, I can only guess. Apart from > using fresh solution (as others have mentioned) also note that the > APS/TEMED system does not work at acidic pH. For such gels you need to > use either a Fenton-system or photopolymerisation. For the latter, > methyleneblue works better than riboflavin. In my Anal. Biochem. paper I > describe both options. > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
