[R] how to merge replicate spots

Hi, Does anybody know how to merge replicate spots in R Bioconductor? I use home-made spotted arrays and every probe is printed in triplicate. I normalize my data using limma package and I do not know how to get mean from these three replicates. Regards, Barbara Cegielska

[R] 'RG' looks like a pre-2.4.0 S4 object: please recreate it

I would like to load ApoAI.RData. During the operation of reading this data an error occurs. There is also a problem with STF file. > library (limma) > load("ApoAI.RData") Warning message: 'RG' looks like a pre-2.4.0 S4 object: please recreate it > objects() [1] "RG" > names(RG) [1] "R" "G

[R] [Fwd: loading SPOT file]

--- Begin Message --- I have problem with loading SPOT files. Sample of my operations in R object > fromfile=TRUE > fext="spot" > rawboxplot=TRUE > flagi="Flags" > > f<-function(x) as.numeric(x$Flags > -99) > targets<-readTargets("ApoAITargets.txt") > > if (fromfile){ + files<-dir(pattern=fext