I would like to load ApoAI.RData. During the operation of reading this
data an error occurs. There is also a problem with STF file.
> library (limma)
> load("ApoAI.RData")
Warning message:
'RG' looks like a pre-2.4.0 S4 object: please recreate it
> objects()
[1] "RG"
> names(RG)
[1] "R" "G" "Rb" "Gb" "printer" "genes" "targets"
> RG$targets
FileName Cy3 Cy5
c1 a1koc1.spot Pool C57BL/6
c2 a1koc2.spot Pool C57BL/6
c3 a1koc3.spot Pool C57BL/6
c4 a1koc4.spot Pool C57BL/6
c5 a1koc5.spot Pool C57BL/6
c6 a1koc6.spot Pool C57BL/6
c7 a1koc7.spot Pool C57BL/6
c8 a1koc8.spot Pool C57BL/6
k1 a1kok1.spot Pool ApoAI-/-
k2 a1kok2.spot Pool ApoAI-/-
k3 a1kok3.spot Pool ApoAI-/-
k4 a1kok4.spot Pool ApoAI-/-
k5 a1kok5.spot Pool ApoAI-/-
k6 a1kok6.spot Pool ApoAI-/-
k7 a1kok7.spot Pool ApoAI-/-
k8 a1kok8.spot Pool ApoAI-/-
> spottypes=readSpotTypes("ApoAISpotTypes.txt")
> spottypes
SpotType ID Name Color
1 cDNA * * black
2 BLANK BLANK * brown
3 Blank Blank * orange
4 Control Control * blue
> RG$genes$Status=controlStatus(spottypes, RG)
Matching patterns for:
Error in matrix(unlist(value, recursive = FALSE, use.names = FALSE),
nrow = nr, :
attempt to set an attribute on NULL
Thank you for your help.
Regars,
B.
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