Paul What kind of cells are these? I have seen a similar problem with plant cells, but never with animal cells. Jay
> -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Phelan, Paul J. > Sent: Monday, April 27, 2015 2:45 PM > To: [email protected] > Subject: Whole cell extract gel samples for western blot > > Sometimes our gel samples (whole cell extracts) for western blots are > very viscous. What are the best methods for lysing small cell > extracts well enough so that they don't float back out of the wells when > loaded? > > Thank you, > > Paul Phelan > Tufts University Boston > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
