Sonication may help. Moreover, try to add NP40 to the sample before the sonication. Good luck!
On Mon, Apr 27, 2015 at 9:44 PM, Phelan, Paul J. <[email protected]> wrote: > Sometimes our gel samples (whole cell extracts) for western blots are very > viscous. What are the best methods for lysing small cell extracts well > enough so that they don't float back out of the wells when loaded? > > Thank you, > > Paul Phelan > Tufts University Boston > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- Anna Feldman, Phd, Laboratory of Computational Systems Biology (Tuller lab), Biomedical Dept, Faculty of engineering, TAU [email protected] 03-6407582 0547606327 _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
