when they are so viscous is due to DNA...try to make a milder lysis..or you can spin them @14K and take the supernatant ..or also sonicate it...
Fulvio Celsi BsC,PhD Immunobiology Lab. *I.R.C.C.S. materno infantile Burlo Garofolo* via dell'Istria, 65/1 34137 Trieste Italy Office +390403785273 Mobile: +393286489131 2015-04-28 13:53 GMT+02:00 Alejandro Miguel Martin Dunn < [email protected]>: > Plain vortexing has worked for me in the past. I have also tried squirting > the sample through a small bore needle (gauge 23) using an insulin syringe, > and it works, but is messier. > > Hope it helps! > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of Phelan, Paul J. > Sent: Monday, April 27, 2015 2:45 PM > To: [email protected] > Subject: Whole cell extract gel samples for western blot > > Sometimes our gel samples (whole cell extracts) for western blots are very > viscous. What are the best methods for lysing small cell extracts well > enough so that they don't float back out of the wells when loaded? > > Thank you, > > Paul Phelan > Tufts University Boston > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
