Hello I am working on recombinant protein UVcrosslinkings to determine the dimerisation sites by sitespecific insertion of pbenzoyl phenyl alanine(crosslinker) into protein in E.coli. After induced, purified the protein, done crosslinking and run SDS Page to check the results. Fortunately Wt didnt show dimer(as expected), only one position(proteinL127pBpa) have shown thickest dimer band rest(each protein has pbpa in various position) shown faint dimer. I suppose not to get faint dimers everywhere where crosslinker available, atleast few should not have shown faint dimer. I am not getting what to do?
Could anyone have suggestions to my problem? Cheer Sudheer -- _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
