I am trying to extract nucleic acids from mucinous tumors and ascites. These samples are predominately mucin2, which contains a proteinase K resistant glycoprotein domain.
I've been using typical methods of extraction, namely proteinase K digestion (which does make the lysate much less viscous), phenol extraction, and ethanol precipitation. Unfortunately, no matter how many times I extract with phenol, there is always material left at the interface, and ethanol precipitation yeilds a pellet that is mostly protein. There is a protease that digests mucin, EhCP5, but it is not commercially available, and I'd like to avoid making my own recombinant proteins. What alternative techniques should I be looking at? -Ed _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
