I have looked at the package .........it won't work for me since it requies
us to read files with the format :
The text file must have the following structure:
1st column = names denoting genes or primer pairs
2nd column = plate index of each gene or primer pair
remaining columns = (replicate) Ct values.
In my case the data I have is like following:
Well Sample Name Detector Task Ct StdDev Ct Qty
Mean Qty StdDev Qty Filtered
Tm User Defined #1 User Defined #2 User Defined #3
A1 4490 ADORA2A Unknown 27.5961 0.236
A2 4490 ADORA2A Unknown 27.1953 0.236
A3 4490 ADORA2A Unknown 27.6102 0.236
A4 5775 ADORA2A Unknown 31.3386 0.296
Andrej Kastrin-3 wrote:
>
> Check out qpcR package.
>
> Andrej
>
> gauravbhatti wrote:
>> Hi I need help since I have never worked on RT PCR data before . I have
>> 8
>> data files each corresponding to a single pcr run. Each file also
>> represent
>> 10 samples(5 cancer and 5 healthy) belonging to a unique gene. I have 7
>> unique genes and 1 reference (total 8 files). Can any body help me with
>> preproceesing and normalization?
>
> ______________________________________________
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> PLEASE do read the posting guide
> http://www.R-project.org/posting-guide.html
> and provide commented, minimal, self-contained, reproducible code.
>
>
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