On Jul 20, 2009, at 6:43 PM, Andrej Kastrin wrote:

Check out qpcR package.

Andrej

gauravbhatti wrote:
Hi I need help since I have never worked on RT PCR data before . I have 8 data files each corresponding to a single pcr run. Each file also represent 10 samples(5 cancer and 5 healthy) belonging to a unique gene. I have 7 unique genes and 1 reference (total 8 files). Can any body help me with
preproceesing and normalization?

Also, try asking this question again on the bioconductor list, as you'll likely find a higher density of people there doing related work.

Some other packages that might help:

  * http://bioconductor.org/packages/2.4/bioc/html/qpcrNorm.html
  * http://bioconductor.org/packages/2.4/bioc/html/SLqPCR.html

-steve

--
Steve Lianoglou
Graduate Student: Physiology, Biophysics and Systems Biology
Weill Medical College of Cornell University

Contact Info: http://cbio.mskcc.org/~lianos/contact

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