You can also use 'dplyr' library(tidyverse) result <- pcr %>% group_by(Gene, Type, Rep) %>% summarise(mean = mean(Ct), sd = sd(Ct), oth = sd(Ct) / sqrt(sd(Ct)) )
Jim Holtman *Data Munger Guru* *What is the problem that you are trying to solve?Tell me what you want to do, not how you want to do it.* On Wed, Mar 27, 2019 at 7:40 PM Jim Lemon <drjimle...@gmail.com> wrote: > Hi Cyrus, > Try this: > > pcr<-data.frame(Ct=runif(66,10,20),Gene=rep(LETTERS[1:22],3), > Type=rep(c("Std","Unkn"),33),Rep=rep(1:3,each=22)) > testagg<-aggregate(pcr$Ct,c(pcr["Gene"],pcr["Type"],pcr["Rep"]), > FUN=function(x){c(mean(x), sd(x), sd(x)/sqrt(sd(x)))}) > nxcol<-dim(testagg$x)[2] > newxs<-paste("x",1:nxcol,sep="") > for(col in 1:nxcol) > testagg[[newxs[col]]]<-testagg$x[,col] > testagg$x<-NULL > > Jim > > On Thu, Mar 28, 2019 at 12:39 PM cir p via R-help <r-help@r-project.org> > wrote: > > > > Dear users, > > i am trying to summarize data using "aggregate" with the following > command: > > > > > aggregate(pcr$Ct,c(pcr["Gene"],pcr["Type"],pcr["Rep"]),FUN=function(x){c(mean(x), > sd(x), sd(x)/sqrt(sd(x)))}) > > > > and the structure of the resulting data frame is > > > > 'data.frame': 66 obs. of 4 variables: > > $ Gene: Factor w/ 22 levels "14-3-3e","Act5C",..: 1 2 3 4 5 6 7 8 9 10 > ... > > $ Type: Factor w/ 2 levels "Std","Unkn": 2 2 2 2 2 2 2 2 2 2 ... > > $ Rep : int 1 1 1 1 1 1 1 1 1 1 ... > > $ x : num [1:66, 1:3] 16.3 16.7 18.2 17.1 18.6 ... > > > > The actual data is "bundled" in a matrix $x of the data frame. I would > like to have the columns of this matrix as individual numeric columns in > the same data frame instead of a matrix, but cant really figure it out how > to do this in an efficient way. Could someone help me with the construction > of this? > > > > Thanks a lot, > > > > Cyrus > > > > ______________________________________________ > > R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see > > https://stat.ethz.ch/mailman/listinfo/r-help > > PLEASE do read the posting guide > http://www.R-project.org/posting-guide.html > > and provide commented, minimal, self-contained, reproducible code. > > ______________________________________________ > R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide > http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code. > [[alternative HTML version deleted]] ______________________________________________ R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.