There is no depth of field, even at moderate magnification. The difference between 'in-focus' and 'out-of-focus' at 1000X is less than 1,5 um. For example when the binocular tube is in good focus the camera tube is so far out of focus that almost no detail can be seen and the difference in height is only 2,5 um. At 100X its slightly better and the range may be as much at 40 um or a bit more -- I'll check.
An RBC is a couple of um thick as it lies on the slide. A neutrophile might be twice, or a little more in thickness. Focus on the neutrophile and the RBC is no longer sharp (at 1000X). However, you can't see this on the pictures I put up because they are so magnified that everything is a bit fuzzy. However, there is a still lot of fine detail to be seen in the white cell nuclei. However, since there is no camera lens involved and no diaphragm to close the DOF is not useful. Don _______________ Dr E D F Williams http://personal.inet.fi/cool/don.williams Author's Web Site and Photo Gallery Updated: March 30, 2002 ----- Original Message ----- From: "Fred" <[EMAIL PROTECTED]> To: <[EMAIL PROTECTED]> Sent: Thursday, June 19, 2003 6:47 AM Subject: Re: More micrographs with ME Super and speed trouble > > An LX would be the perfect solution. > > Plus, compared to the ME Super, the LX has a DOF preview lever - you > really want to checkout that "huge" DOF at 1570X - <VBG>. > > Fred > > >
