I had found a simple centrifugal extrusion of the DNA from the gel to be gentler and less costly.
Hiranya S. Roychowdhury, Ph.D. Professor Department of Science Division of Science, Engineering and Math NMSU-Dona Ana Community College 575 527 7725 (office) Curriculum & Instruction Committee Human Anatomy and Physiology Society<http://www.hapsweb.org/> [http://www.hapsweb.org/graphics/header.jpg] ________________________________ From: [email protected] <[email protected]> on behalf of DK <[email protected]> Sent: Thursday, November 9, 2017 5:25:54 PM To: [email protected] Subject: Re: Gel Solubilization Buffer Composition In article <[email protected]>, [email protected] wrote: >On Saturday, 9 January 2010 18:42:04 UTC, wattne wrote: >> On 9 Jan., 16:40, Jagadish Katam <[email protected]> wrote: >> > Hi, >> > >> > I am working on purifying the DNA by gel extraction procedure and are using > the commercially available kits for this purpose. A yellow color gel > solubilization buffer is used to dissolve the sliced gel. >> > >> > Here i would know the composition of this gel solubilization buffer. >> > >> > Any suggestions will be greatly appreciated. >> > >> > Thanks and Best Regards, >> > Jag >> >> Hi Jag! >> Sounds like the buffer from QIAGEN, right? Why aren't you more >> precise?? How should anyone help you if we don't even know what kind >> of stuff you are using??? >> You can bet that the buffer composition is secret because otherwise >> QIAGEN couldn't sell their kit anymore; what do you think? Try a >> Google, and you will either find out or not. Why should we know the >> exact formulation???? >> Thanks and Best Regards, >> watnne > >Hi Watnne > >I found all the other replies to Jag's question helpful and constructive. Yours > felt fairly malicious. Please refrain from comments like this in the future, > its a waste of time and damages the community feeling of places like this. I don't know about thje feelings but here is the composition: 5.5 M guanidine thiocyanate, 20 mM Tris-HCl, pH 6.6 DK _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
