On Jan 9, 11:40 am, Jagadish Katam <[email protected]> wrote: > Hi, > > I am working on purifying the DNA by gel extraction procedure and are using > the commercially available kits for this purpose. A yellow color gel > solubilization buffer is used to dissolve the sliced gel. > > Here i would know the composition of this gel solubilization buffer. >
In general, agarose gel solubilization can be done with chaotropic salts such as NaI, sodium perchlorate, or guanidine HCl. These also work well in conjunction with silica-based binding resins because DNA binding to silica is promoted in high salt. I doubt the company will tell you exactly what is in their buffer, but it is likely similar to one the salts I mentioned, possibly with additives added as a stabilizer or other use. Nick -- Nick Theodorakis [email protected] contact form: http://theodorakis.net/contact.html _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
