Try benchling.com, a lot better than zhang lab site On Wed, Oct 5, 2016 at 9:25 AM, <[email protected]> wrote:
> Hi, > I am new to CRISPR/Cas9. I tried to use the link > http://crispr.mit.edu/guides/3386043089646727 > to design gRNAs for my gene of interest. > > It came out a lot of off target sites for PX458. None of them has no off > target. > number of offtarget sites: 132 (18 are in genes) > > My question is normally how would you pick the gRNAs is there a criteria > for selecting the gRNAs. > > Thanks, > ZJ > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
