[email protected] (DK) wrote: >293T cells do *not* lack any metabolic pathways. "T" stands for large T antigen >of SV40 virus (=replication factor for circular DNA that contains SV40 ori). >They >require no conditional medium and no extra additives beyond what is normally >added to any 293 culture.
Additionally people should keep in mind that due to this transformation the cells already carry a selective marker and are resistant to neomycin/G418. Christian -- X-no-Sig: yes _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
