On Tuesday, December 16, 1997 11:30:00 AM UTC+3:30, Warren Hirst wrote: > I have used IBMX, as a phosphodiesterase inhibitor, in cAMP accumulation > assays. The final concentration used was 500uM (micromolar) in the assay > medium (see Hirst et al., (1997) British Journal of Pharmacology 120, > 509-515). This concentration was achieved by dissolving 5.5 mg of IBMX > (Sigma) in 200ul of DMSO and then adding this to 50ml of media. > Hope that this has been of some help. > > Warren.
Dear Warren, Based on Sigma, The dilution solution should be prepared in "Warm DMSO". But How Much warm. Thank you. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
