> Message: 3 > Date: Mon, 12 Apr 2010 18:14:48 -0400 > From: Ruiying Wang <[email protected]> > Subject: why do we only use ?-[32P]ATP to radiolable RNA? > To: [email protected] > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=ISO-8859-7 > > Hi, > > I have been using ã-[32P]ATP to radiolable my RNA, but I can not find the > reason why we only use ã-[32P]ATP to radiolable RNA, why do not use other > ã-[32P]NTPs? > > Thanks, > > Ruiying
Hello We make RNA probes using PCR with [32P]dCTP in the mix for labelling. So far it has worked great, always. This is actually the only dNTP we use for the labelling, but I don't discard its posible to use others. P. > ------------------------------ > > > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
