Simulating cancer genomes

Yevgeniya Nusinovich

Most studies of cancer genomics analyze existing tumors, probing their
genetic sequence for clues as to what mutations drive their initiation and
progression. However, this approach requires access to patient materials,
so it presents privacy issues and may be limited by the availability of
tumors with specific mutation patterns. As an alternative, Díaz-Navarro et
al. used generative AI trained on databases of existing cancer genomes to
create synthetic ones that mimic real tumors. It remains to be seen whether
these synthetic cancer genomes can help to uncover new tumor biology, and
there are some types of genetic damage that they cannot replicate yet, but
this orthogonal approach may yield biological insights.

Synthetic Biology

Exploring multicellularity with yeast

L. Bryan Ray

Yeast lack the specific adhesion interactions and cell contact–dependent
signals that allow more complex processes in multicellular organisms. With
an eye to enabling synthetic biology applications, Meng et al. explored
whether yeast cells could be programmed to exhibit multicellular behaviors.
They engineered Saccharomyces cerevisiae cells to express surface proteins
that mediate signaling between cells that come into physical contact. The
toolkits that they developed allowed control of cell adhesion and
consequent signaling between cells to create multicellular logic circuits
that recapitulate cellular interactions in the development of multicellular
organisms. The authors designed a sensor for protein-protein interactions
and anticipate other applications in biotechnology.

Ancient DNA from cave sediments gives a rare glimpse of Norwegian coastal
fauna before the Last Glacial Maximum.

Although deep-time paleogenomic studies have increased in recent years,
preserving organic remains requires specific circumstances. Glacier melting
and erosion can disperse organic material, leaving behind only rock. Walker
et al. performed stratigraphic analysis in a cave in northern Norway with
geomorphological features that allowed for draining of glacial meltwater
without sediment layer disruption. These sediments predate the Last Glacial
Maximum and are likely about 71,000 to 85,000 years old. Bulk-bone
metabarcoding revealed DNA from 46 animal taxa encompassing birds, mammals,
and fish, as well as pollen and marine invertebrates. These data grant a
previously unseen snapshot of this Arctic coastal environment, including
the identification of a now extinct species that has been rarely documented.

Little red dots (LRDs) are a class of distant galaxies that are compact,
red, and lack x-ray emission. Their physical nature is debated, but one
possibility is that they are active galaxies with the supermassive black
hole (SMBH) in their nuclei obscured by surrounding gas. Taylor et al.
observed an LRD at redshift 9.2, about 500 million years after the Big
Bang. Spectral line analysis showed that it contains a surprisingly massive
SMBH, over 5% of the stellar mass of the galaxy, and is seemingly
surrounded by very dense gas. It remains unclear how such a massive SMBH
could have formed so early or how the high gas density could be maintained.

Self-assembled islands of C60 on graphene create well-defined hole-doped
regions that act as mid-infrared plasmonic cavities. Rizzo et al. thermally
evaporated C60 onto graphene to form clusters ranging from 50 to 300
nanometers in diameter. Scattering-type scanning near-field optical
microscopy revealed high-contrast surface plasmon polariton cavity modes,
as verified by energy-dependent measurements and optical modeling. The
surface plasmon polariton wavelength could be tuned by altering the
deposition conditions to change the cluster sizes.

During the pathogenesis of Alzheimer’s disease, some neurons become
pathologically hyperactive, whereas others turn silent. Although neuronal
hyperactivity has been extensively studied, Zhang et al. investigated the
mechanisms underlying neurons becoming virtually silent in Alzheimer’s
disease. Combining awake two-photon imaging, single-neuron intracellular
recordings, and single-neuron trans-synaptic tracing followed by
post-mortem large-scale imaging of presynaptic neurons, the authors
identified hyperactive or silent cells in vivo, recorded their subthreshold
firing patterns, and conducted post-mortem tracing of the neuron’s
presynaptic inputs. Silent neurons showed a profound loss of both
excitatory and inhibitory inputs compared with hyperactive neurons, which
exhibited surprisingly few changes. This synaptic decoupling may play an
important role in the earliest stages of Alzheimer’s disease.

Student evaluations of teaching are gender biased, and interventions for
mitigating these biases are being studied. Mitchem et al. investigated
whether the introduction of a simple AntiBias statement to student
evaluations could mitigate bias toward instructor gender within the context
of a biology department. Over the course of three semesters, students were
given either the original evaluation or one containing the AntiBias text.
Results were mixed, with the AntiBias treatment inconsistently influencing
student evaluations. Qualitative analysis revealed that the students’
comments were mostly not affected by the conditions of treatment or
instructor gender, although students receiving the AntiBias treatment wrote
more. These results suggest that mitigating gender bias in teaching
evaluations is nuanced and will likely not result from a simple
intervention.

CBE Life Sci. Educ. (2025) 10.

K Rajaram IRS  31025

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