[R] How to find quantitative cycle PCR with R?

Hello, I would like to analyze quantitative PCR (qPCR) data using R rather than proprietary softwares. I can see there are several packages out there, but mostly they perform relative quantification, while none provide a quantitative cycle (Cq), or crossing over cycle. Is there a package that can p

Re: [R] Estimating regression with constraints in model coefficients

there are ways to implement constraints on parameter estimates in ordinal logistic regression in R. Here are a few approaches: The rms package (Regression Modeling Strategies) by Frank Harrell offers the lrm function which can handle constraints through its penalty parameter, though it's primar

[R] Estimating regression with constraints in model coefficients

Hi, I have below fit with ordinal logistic regression dat = foreign::read.dta("https://stats.idre.ucla.edu/stat/data/ologit.dta";) summary(MASS::polr(formula = apply ~ pared + public + gpa, data = dat)) However, instead of obtaining unconstrained estimates of model parameters, I would like to i

Re: [R] An opinion question, please

I was thinking about xts and zoo as well! Thanks! Erin Hodgess, PhD mailto: erinm.hodg...@gmail.com On Tue, Apr 8, 2025 at 12:39 AM Eric Berger wrote: > If you are writing a new package I don't see why you should restrict > yourself to S3 or S4 classes. > Your "new package" can certainly buil

Re: [R] An opinion question, please

I would use the approach that is most commonly used by my potential user base unless I can define a clear set of reasons why I should do it differently. If users will be unable to see the difference, then the decision is entirely up to me based on ease of programming at all phases (writing, debu

Re: [R] An opinion question, please

If you are writing a new package I don't see why you should restrict yourself to S3 or S4 classes. Your "new package" can certainly build on other packages. (Why not?) As I wrote in my previous email, if you are dealing with time series you might want to consider appropriate classes already defined

Re: [R] An opinion question, please

Às 18:35 de 07/04/2025, Erin Hodgess escreveu: Hello everyone! I have an opinion question please. If I’m writing a new package, would you recommend using S3 or S4 structure, please? I know I will get lots of opinions, but that’s fine. Thanks, Erin Erin Hodgess, PhD mailto: erinm.hodg...@gma