Hope it helps.
text <- "var1 var2
9G/G09 abd89C/T90
10A/T9 32C/C
90G/G A/A"
x <- read.table(textConnection(text), header = T)
x$var1.1 <- sub(".*(.)/.*", "\\1", x$var1)
x$var1.2 <- sub(".*/(.).*", "\\1", x$var1)
x$var2.1 <- sub(".*(.)/.*", "\\1", x$var2)
x$var2.2 <- sub(".*/(.).*", "\\1", x$var2)
-----
A R learner.
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