I don't know any about you research but I agree with Gabor: the model is identifiable.
No*(1-exp(a*(b*Ne-T))) can be reparametrized to No*(1-exp(C*NeD)), where C=a*b and D=a*T. This reduces the model to 3 parameters and can see shown that is a reperametrization of the SSasympOff() defined in R (with default start values). "No" is a parameter too, so you need provide start values in start list. Look: # indentifiability No <- 100; a <- 1; b <- -1; T <- 2 Ne <- seq(1, 10, l=8) curve(No*(1-exp(a*(b*x-T))), 0, 10) abline(h=No*(1-exp(a*(b*0-T)))) # intercept C <- a*b; D <- a*T curve(No*(1-exp(C*x-D)), add=TRUE, lty=2, col=2, lwd=2) help(SSasympOff, help_type="html") # model speficication nls(Ne~No*(1-exp(a*(b*Ne-T))), start=list(a=1.2, b=0.015, T=24)) ^ ^ ^ ^ y y? > y <- No*(1-exp(a*(b*Ne-T)))+rnorm(Ne,0,0.1) > plot(y~Ne) > nls(y~No*(1-exp(a*(b*Ne-T))), start=list(No=No, a=a, b=b, T=T)) Erro em numericDeriv(form[[3L]], names(ind), env) : Obtido valor faltante ou infinito quando avaliando o modelo > nls(y~No*(1-exp(C*Ne-D)), start=list(No=No, C=C, D=D)) Nonlinear regression model model: y ~ No * (1 - exp(C * Ne - D)) data: parent.frame() No C D 99.9763 -0.9972 2.0197 residual sum-of-squares: 0.02746 Number of iterations to convergence: 2 Achieved convergence tolerance: 6.673e-07 > Bests. ----- ..ooo0 ................................................................................................... ..(....)... 0ooo... Walmes Zeviani ...\..(.....(.....)... Master in Statistics and Agricultural Experimentation ....\_)..... )../.... walmeszevi...@hotmail.com, Lavras - MG, Brasil ............ (_/............................................................................................ -- View this message in context: http://r.789695.n4.nabble.com/NLS-Singular-Gradient-Error-tp2069029p2073452.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.