On Feb 28, 2010, at 5:33 PM, Martin Batholdy wrote:

Hi,

which test do I have to use if I want to test if the following data follow a monotone trend;

min     5min    10min   20min   30min
5               20              55              70              90

... where the dependent variable contains frequencies.

It is not clear what you mean by "frequencies". Do you mean counts? I's also unclear what your data situation is and what sort of independence assumptions might be met (or not). Here's a possible computational run that might have a plausible error structure for count data and form the basis for a "linear trend test" but whether it can be applied to your experiment is not assured. In particular the independence assumption seems questionable if the counts are arising from the same experimental subjects. You may want to calculate the differences if these are cumulative counts:

> dta <-data.frame(time=c(1,5,10,20,30), counts=c(5,20,55,70, 90))

> glm(counts~time, data=dta, family="poisson")

Call:  glm(formula = counts ~ time, family = "poisson", data = dta)

Coefficients:
(Intercept)         time
    2.92950      0.05704

Degrees of Freedom: 4 Total (i.e. Null);  3 Residual
Null Deviance:      123.3
Residual Deviance: 32.91        AIC: 63.51

If these are cumulative counts, the "monotone trend" may not be as solidly supported:

> dta$diffs <-c(dta$counts[1], diff(dta$counts))
> dta
  time counts diffs
1    1      5     5
2    5     20    15
3   10     55    35
4   20     70    15
5   30     90    20
> mod0 <- glm(diffs~1, data=dta, family="poisson")
> mod <- glm(diffs~time, data=dta, family="poisson")
> anova(mod,mod0)
Analysis of Deviance Table

Model 1: diffs ~ time
Model 2: diffs ~ 1
  Resid. Df Resid. Dev Df Deviance
1         3     25.034
2         4     27.014 -1  -1.9799




--
David Winsemius, MD, MPH

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