Hello Martial,
Cell segmentation is not easy in many cases. Cells may overlap and
contain complex structures such as organelles or cytoskeletal fibers
that hinder the determination of cell membranes.
There is no one-size-fits-all algorithm that can process and analyse
cellular tissues in an automatic way. It really depends on your cells
and stains.
The package EBImage provides general purpose functionality
for reading, writing, processing and analysis of images. EBImage
documentation contains an example on how to segment, quantify and
classify HeLa cells in different states:
http://www.bioconductor.org/packages/release/bioc/html/EBImage.html
Hope this will help,
Greg
---
Gregoire Pau
EMBL Research Officer
http://www.ebi.ac.uk/~gpau/
Martial Sankar wrote:
Hi,
I am looking for a R package which is capable to process and analysis pictures
of tissues (stained) in an automatic way.
I had a look on biops and EBImage (Bioconductor) but they are not automatic...
Did you already use/know a such package ?
Thanks,
- Martial
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