Hi all, I have loaded the LIMMA and Biobase package and tried these commands: library(limma) library("Biobase") data <- read.table("c:/temp/data.txt",header=T,row.names=1) ExpressionData <- as.matrix(data[,c(2,3,4,6,7,8)]) eset <- new("ExpressionSet", exprs = ExpressionData) design <- cbind(WT=1,P=c(0,1,1,0,1,1),G=c(0,1,0,0,1,0)) fit <- lmFit(eset,design) But I keep getting an eroor message after "lmFit" that says: Error in possibleExtends(cl, class2, class1Def, class2Def) : trying to get slot "subclasses" from an object (class "classRepresentation") that is not an S4 object I am using R-2.9.2. Whats happening now? I have done exactly the same commands in another computer and it works fine. I forgot which R version it was. But after installing the latest version nothing works. Pls advice... Regards, Wendy
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