Hi I have some experimental data where I have counts of the number of insects collected to different trap types rotated through 5 different location (variable -location), 4 different chemical attractants [A, B, C, D] were applied to the traps (variable - semio) and all were trialled at two different CO2 release rates [1, 2] (variable CO2) I also have a selection of continuous variables measuring meteorological conditions to account for any bias cause by changing weather conditions, the data is over dispersed so I have fitted a negative binomial glm (glm.nb) and simplified using stepAIC from the MASS package etc. There are significant differences in the number of insects attracted to the different chemical (semio) and to the two different CO2 (release rates) I have then used the glht function from the multcomp package to do multiple comparisons to see what the specific differences between the levels are for semio and CO2 using the code below which works great but what I would like to do is to do comparisons combining the factors e.g a comparison for semioA at CO at level 1 vs Semio A at CO2 level 2 etc to see which is the best combination, is this possible or should I have started of with my counts already split up into this e.g. a treatment variable(semioA at CO2 level 1 = A1, semioA at CO2 level 2 = A2 etc), I started with them this way as we have no prior knowledge that increasing co2 will have any effect. I have had a quick try with the data split into a treatment factor (instead of semio and CO2 level) but I can not get convergence with glm.nb I think this may be to do with to many zeros in the data set, do you know if glht or another multiple comparison will work or zeroinflated negative binomial regression(zeroinfl() from the pscl library)? Any help or ideas will be gratefully appreciated. Many thanks in advance. Lara semiochemical <-read.csv("G:/semiochemical_data.csv", header=T)
semiochemical$location2<-factor(semiochemical$location) levels(semiochemical$location2)<-c("1","2","3","4","5") semiochemical$semio2<-factor(semiochemical$semio) levels(semiochemical$semio2)<-c("A","B","C","D") semiochemical$CO22<-factor(semiochemical$CO2) levels(semiochemical$CO22)<-c("1","2") model1<-glm.nb(total ~ semio + CO2 + location + temp + mean.wind.speed) model.glht.Semio <- glht(model1, linfct=mcp(semio="Tukey")) model.glht.CO2 <- glht(model1, linfct=mcp(CO2="Tukey")) [[alternative HTML version deleted]] ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.