<<Barplot_qpcr_Isabella_Dce08_SL_cal5.r>>  <<qpcr_barplot.txt>>  
<<plot2_JS-W2_HKG21-Cal5.pdf>> 

I attached the R code,  data and plot. I am wondering why I couldn't get
subtitle showing up under each plot even though I didn't get any error
message. 

Thanks for help in advance,
Shaorong
Sample  NKa1b_2 NKa1c_2 NKAa3_2 NKAb1_1 Group
1556    -0.14928982     -0.146699076    -0.022236441    -4.046102264    JS_HH
1626    -0.170271084    0.049696065     -0.225277585    0.851861452     JS_HH
1677    -0.023359567    0.054504736     0.299314951     0.975459979     JS_HH
1745    -0.280403029    -0.110558584    -0.058096141    0.643061398     JS_HH
1322    0.277170917     -1.018182797    0.153302613     -4.125755573    JS_UH
1355    0.029640828     0.013059459     0.10176975      0.73826325      JS_UH
1376    0.537833924     0.498861689     0.822027081     0.275237161     JS_UH
1404    -0.072231121    -0.009676466    0.081658873     0.529725597     JS_UH
1421    0.269019997     0.315229777     0.164296186     0.978087392     JS_UH
1448    0.423323368     0.325610763     0.188268333     0.604567952     JS_UH
1520    -0.152175597    -0.088982454    -0.00317745     0.694865259     JS_UH
1637    0.279098956     0.09674064      -0.60969932     0.969939918     JS_UH
1740    -0.305083125    0.162734477     0.139792974     -1.701646147    JS_UH
1769    0.013103623     0.090727937     0.051869886     0.919403273     JS_UH
24      -0.100648488    0.081747891     -0.091458258    -4.244210716    W_HH
34      0.041359599     -0.043213716    -0.047314396    -4.15756455     W_HH
83      -0.118185336    0.004305679     -0.044859266    -3.163651353    W_HH
98      -0.088802214    -0.056019396    -0.191949942    0.733591243     W_HH
122     -0.200487325    -0.209960419    -0.264005258    0.882023718     W_HH
144     0.263879466     0.311560822     0.1009223       0.407358097     W_HH
228     0.165844087     0.179544409     0.053720993     0.756049833     W_HH
308     -0.13334169     0.059431671     0.053755453     0.684082343     W_HH
362     0.137440519     0.279497504     0.161421911     -4.165294587    W_HH
75      0.16323564      0.119034461     0.217261002     0.84941149      W_UH
167     0.472053669     0.400633437     0.293564648     0.931563634     W_UH
193     0.358867692     0.420990019     0.263630909     0.649366339     W_UH
210     0.281419172     0.29477039      0.282901177     0.769709346     W_UH
214     0.272700428     0.312086763     0.345958722     -3.753016482    W_UH
237     0.183989578     0.2108481       0.208665138     0.574668113     W_UH
302     -0.270415946    -0.11194806     -0.196944588    0.814442285     W_UH
315     0.569414972     0.615197576     0.289420307     -0.059768719    W_UH
329     -0.040500835    -0.030366091    -0.204481188    0.944737301     W_UH
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