Dear all

Sorry, this is probably the most off-topic mail I have ever sent to this help 
list. However maybe somebody could point me to right direction or give some 
advice.

In microscopy particle counting you have finite viewing field and some 
particles could be partly outside of this field. My problem/question is:

Do bigger particles have also bigger probability that they will be partly 
outside this viewing field than smaller ones?

Saying it differently, although there is equal count of bigger (white) and 
smaller (black) particles in enclosed picture (8), due to the fact that more 
bigger particles are on the edge I count more small particles (6) than big (4).

Is it possible to evaluate this feature exactly i.e. calculate some bias 
towards smaller particles based on particle size distribution, mean particle 
size and/or image magnification?

Best regards
Petr Pikal
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Attachment: particle.pdf
Description: particle.pdf

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