> On May 27, 2016, at 10:07 AM, James Henson <jfhens...@gmail.com> wrote: > > Greetings Jeff, > You are correct that the unequal number of levels is not the problem. > I revised the data frame so that the number of levels was equal and > the same error message occurred. The code is below, and the > Eboni2.txt file is attached. This problem baffles me. I appreciate > any help. > Best regards, > James > Eboni2 <- read.csv("Eboni2.csv", header = TRUE) > > library("nlme") > > str(Eboni2) > > head(Eboni2) > > model1 <- lme(preDawn ~ Irrigation, random=~season_order|treeNo, data=Eboni2)
I downloaded the attached file to your first posting that was called a "csv" file but it was tab-separated (as could be clearly seen with the str output, so would only load properly with read.delim rather than read.csv. Running then with the lme call, it produced this message > model1 <- lme(preDawn ~ Irrigation, random=~season_order|treeNo, data=Eboni2) Error in na.fail.default(list(season_order = c(5L, 5L, 5L, 5L, 5L, 5L, : missing values in object And looking at the str result made it clear that there were many NA's in the file. > head(Eboni2) number Location Season season_order Month treeID treeNo preDawn midday 1 1 UCC November 5 Nov UCCLO 1 60 1.4 1.3 2 2 UCC November 5 Nov UCCLO 2 72 1.2 1.3 3 3 UCC November 5 Nov UCCLO 3 78 1.1 1.2 4 4 UCC November 5 Nov UCCLO 4 79 1.1 2.1 5 5 UCC November 5 Nov UCCLO 5 80 1.4 1.3 6 6 UCC November 5 Nov UCCLO 6 81 0.6 1.8 Irrigation Pnet Gs E WUE d15N d13C Nper Cper include2 1 N 9 0.2907004 3.766207 2.389672 NA NA NA NA no 2 N 11 0.3262582 3.120574 3.524993 NA NA NA NA no 3 N 8 0.2870957 1.693821 4.723050 3.00 -27.44 2.12 52.12 yes 4 N 10 0.2475180 1.839343 5.436724 3.61 -29.50 1.42 51.97 yes 5 N 13 0.3009228 3.082278 4.217660 NA NA NA NA no 6 N 17 0.3487337 2.534550 6.707304 2.79 -30.50 1.49 49.94 yes And even more importantly, there was one NA in your outcome variable: > sum( is.na(Eboni2$Irrigation)) [1] 0 > sum( is.na(Eboni2$preDawn)) [1] 1 So after restricting to complete.cases, I then formed the hypothesis that you reversed the order of the variables in the formula for the random parameter: > table(Eboni2$season_order) 1 2 3 4 5 83 83 83 83 83 > length( Eboni2$treeNo) [1] 415 So it seemed unreasonable to have a "grouping" on variable with only one item per group. > model1 <- lme(preDawn ~ Irrigation, random=~treeNo|season_order, data=Eboni2[ > complete.cases( Eboni2[ , > c('preDawn','Irrigation','season_order','treeNo')]), ] ) > model1 Linear mixed-effects model fit by REML Data: Eboni2[complete.cases(Eboni2[, c("preDawn", "Irrigation", "season_order", "treeNo")]), ] Log-restricted-likelihood: -183.4708 Fixed: preDawn ~ Irrigation (Intercept) IrrigationY 1.04520145 -0.06037706 Random effects: Formula: ~treeNo | season_order Structure: General positive-definite, Log-Cholesky parametrization StdDev Corr (Intercept) 0.140239324 (Intr) treeNo 0.003766019 -0.725 Residual 0.365678898 Number of Observations: 414 Number of Groups: 5 (Warning, I'm not a frequent user of this package or any of the mixed effects packages.) Just to correct some misinformation that appeared earlier: You can attach "csv" or "tsv" files as long as you name them with an .txt extension so the mail clients and servers consider them to be MIME-text. -- David. > On Wed, May 25, 2016 at 6:23 PM, Jeff Newmiller > <jdnew...@dcn.davis.ca.us> wrote: >> Please keep the mailing list in the loop by using reply-all. >> >> I don't think there is a requirement that the number of levels is equal, but >> there may be problems if you don't have the minimum number of records >> corresponding to each combination of levels specified in your model. >> >> You can change the csv extension to txt and attach for the mailing list. Or, >> better yet, you can use the dput function to embed the data directly in your >> sample code. >> >> Also, please learn to post plain text email to avoid corruption of R code by >> the HTML formatting. >> -- >> Sent from my phone. Please excuse my brevity. >> >> On May 25, 2016 2:26:54 PM PDT, James Henson <jfhens...@gmail.com> wrote: >>> >>> Good afternoon Jeff, >>> The sample sizes for levels of the factor "Irrigation" are not equal. If >>> 'nlme' requires equal sample sizes this may be the problem. The same data >>> frame runs in 'lme4' without a problem. >>> >>> Best regards, >>> James >>> >>> >>> On Wed, May 25, 2016 at 3:41 PM, James Henson <jfhens...@gmail.com> wrote: >>>> >>>> Good afternoon Jeff, >>>> >>>> When working with this data frame, I just open the .csv file in R Studio. >>>> But, we should not send .csv file to R_help. What should I send? >>>> >>>> Best regards, >>>> James >>>> >>>> On Wed, May 25, 2016 at 2:52 PM, Jeff Newmiller >>>> <jdnew...@dcn.davis.ca.us> wrote: >>>>> >>>>> You forgot to show the commands to us that you used to read the data in >>>>> with (your example is not "reproducible"). This step can make all the >>>>> difference in the world as to whether your analysis commands will work or >>>>> not. >>>>> -- >>>>> Sent from my phone. Please excuse my brevity. >>>>> >>>>> On May 25, 2016 11:59:06 AM PDT, James Henson <jfhens...@gmail.com> >>>>> wrote: >>>>>> >>>>>> Greetings R community, >>>>>> >>>>>> My aim is to analyze a mixed-effects model with temporal >>>>>> pseudo-replication >>>>>> (repeated measures on the same experimental unit) using ‘nlme’. >>>>>> However, >>>>>> my code returns the error message “Error in na.fail.default’, even >>>>>> though >>>>>> the data frame does not contain missing values. My code is below, and >>>>>> the >>>>>> data file is attached as ‘Eboni2.txt. >>>>>> >>>>>> library("nlme") >>>>>> >>>>>> str(Eboni2) >>>>>> >>>>>> head(Eboni2) >>>>>> >>>>>> model1 <- lme(preDawn ~ Irrigation, random=~season_order|treeNo, >>>>>> data=Eboni2) >>>>>> >>>>>> I am genuinely confused. Hope someone can help. >>>>>> >>>>>> Best regards, >>>>>> >>>>>> James F. Henson >>>>>> >>>>>> ________________________________ >>>>>> >>>>>> R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see >>>>>> https://stat.ethz.ch/mailman/listinfo/r-help >>>>>> PLEASE do read the posting guide >>>>>> http://www.R-project.org/posting-guide.html >>>>>> and provide commented, minimal, self-contained, reproducible code. >>>> >>>> >>> >> > > ______________________________________________ > R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see > https://stat.ethz.ch/mailman/listinfo/r-help > PLEASE do read the posting guide http://www.R-project.org/posting-guide.html > and provide commented, minimal, self-contained, reproducible code. David Winsemius Alameda, CA, USA ______________________________________________ R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.