On Thu, May 15, 2008 at 9:22 AM, Luis Cayuela <[EMAIL PROTECTED]> wrote:
> Hi everybody,

> I am trying to fit a model with the lmer function for mixed effects. I have 
> an experimental design consisting of 5 field plots. Each plot is divided in 
> 12 subplots where the influence of three factors on the growing of tree 
> seedlings is tested: (1) seed (1 = presence; 0 = absence); (2) seedling 
> species (oak holm vs. pine); (3) treatment (three different treatments). In 
> each of these subplots we planted 13 seedlings. Therefore I would have a 
> model with three fixed factors and one random factor (a block?). If I´m not 
> wrong the model would be as follows:

> model2 <- lmer(Growing ~ Seed + Species + Treatment +(Seed + Species + 
> Treatment|Block), data)

That's unlikely. This specification would fit 5 fixed effects
parameters and 5, possibly correlated, random effects for each level
of the Block factor.  This would require estimating a total of 15
variance-covariance parameters for the random effects from the 5
blocks.

Can you indicate how many random effects you expect to obtain and how
many variance-covariance parameters would be involved?  For example, a
model with a simple random effect would be expressed as

 lmer(Growing ~ Seed + Species + Treatment + (1|Block), data)

and would involve estimating the 5 fixed effects and one variance for
the random effects.
> My first question is: if the three fixed factors occur within-subjects 
> (considering the plot as a subject), is the model correctly defined (assuming 
> no interactions)? Should I specify the model in some other way?
>
>
>
> I second problem I had is that the factors are not crossed because some of 
> the seedling died during the experiment. This means that some factors are 
> nested. Specifically Species is nested within Seed and Block would be nested 
> within Treatment. I have tried to use the %in% specification for nested 
> designs but it does not work.
>
>
>
> model2 <- lmer(Growing ~ Seed + Species%in%Seed + Treatment +(Seed + Species 
> + Treatment|Block%in%Treatment), data)
>
>
>
> I get the following error:
>
>
>
> Error en lmer(Growing ~ Seed + Species %in% Seed + Treatment +  : ..
>
>  Leading minor of order 5 in downdated X'X is not positive definite
>
>
>
> I would appreciate some help to fit this model.
>
>
> Thanks to everybody,
>
> Luis
>
> Luis Cayuela
> Departamento de Ecología
> Universidad de Alcalá
> Crta. de Barcelona km. 33,600
> E-28871 Alcalá de Henares
> Madrid
> España
> Tlf: (+0034) 918856407
> Fax: (+0034) 918854929
>        [[alternative HTML version deleted]]
>
>
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>

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