Hello
I have done a cluster analysis, and I would like to change the font size of my
sample names. I have tried using cex, but this doesn't work/change anything. I
have tried to specify it with cex.main, cex.lab, cex.axis and cex.sub, and can
change all other names in the picture but my sample names.
I can't see what I am doing wrong.
I hope somebody can help me, and I apologize for this probably very simple
question/mistake.
I have around 1000 rows and 50 columns, but have only included a few in my
example.
Thank you in advance!
Kind regards
Gitte Andersen
#Read in data
data<-read.table("/Users/gban/Desktop/Heatmap/Heatmap with selected
genes/Probes_for_heatmap_35_meth_diff_both_hypo_and_hypermeth_Gene&probenames.txt",sep="\t",dec=",",header=TRUE,row.names=1)
data
OS_Tumor1_08_14985_2_3 OS_Tumor2_08_226869_1
CHST3_cg04268405_1 0.95038060 0.76433753
DLX5_cg19962750_2 0.93111825 0.75384523
ZIC4_cg12892506_3 0.86033747 0.69614933
DLX5_cg05597836_4 0.90698171 0.66414891
#Turn the data into a matrix, and transpose to get the columns to be clustered
Data_matrix<-as.matrix(t(data))
Data_matrix
CHST3_cg04268405_1 DLX5_cg19962750_2
OS_Tumor1_08_14985_2_3 0.9503806 0.9311183
OS_Tumor2_08_226869_1 0.7643375 0.7538452
OS_Tumor3_10_201917_2_3 0.7109182 0.7778035
OS_Tumor4_00_2395 0.7772400 0.6769241
OS_Tumor5_02_2669 0.9638739 0.9023436
OS_Tumor6_02_4738 0.9028490 0.9586764
OS_Tumor7_02_4850 0.8786524 0.8872261
OS_Tumor8_02_6935 0.8434550 0.7180251
OS_Tumor9_03_1430 0.7494400 0.9190213
OS_Tumor10_03_1701 0.9148253 0.7692125
OS_Tumor11_03_220 0.9270112 0.8607459
OS_Tumor12_03_2558 0.9344832 0.5013390
OS_Tumor13_03_373 0.9549153 0.9559071
OS_Tumor14_03_82 0.9117558 0.3993953
OS_Tumor15_06_22319 0.9580999 0.9645215
OS_Tumor16_07_16581 0.6213243 0.9033265
OS_Tumor17_07_28523 0.9064597 0.4421651
OS_Tumor18_07_3212 0.6439032 0.4344106
OS_Tumor19_07_6990 0.9350585 0.9238712
OS_Tumor20_07_6990sample2 0.9526839 0.9027684
OS_Tumor21_07_7724 0.9338156 0.8335415
OS_Tumor22_08_10238 0.9028336 0.8529122
OS_Tumor23_08_14985Sample2 0.9609952 0.9478541
OS_Tumor24_08_16592 0.9168102 0.9138002
OS_Tumor25_08_21197 0.9148711 0.9109843
OS_Tumor26_08_21197Sample2 0.9583610 0.8830407
OS_Tumor27_08_222863 0.9478853 0.8395278
OS_Tumor28_08_225814 0.9451414 0.9407933
OS_Tumor29_08_226869Sample2 0.9028336 0.8337000
OS_Tumor30_08_230660 0.9599117 0.9363075
OS_Tumor31_08_4485 0.8247909 0.8336728
OS_Tumor32_09_214654 0.9531564 0.8957473
OS_Tumor33_09_214654Sample2 0.9552210 0.9034464
OS_Tumor34_10_200150 0.9332627 0.8551589
OS_Tumor35_10_201917 0.9397219 0.9470733
OS_Tumor36_10_201917Sample2 0.9607446 0.9523402
OS_Tumor37_10_202221 0.9264254 0.9413189
OS_Tumor38_10_204294 0.8388658 0.8956207
OS_Tumor39_10_204294Sample2 0.8071109 0.8474199
OS_Tumor40_10_205933 0.8788835 0.4950936
OS_Tumor41_10_225662 0.9334545 0.8910134
OS_Tumor42_10_229129 0.5872184 0.8394597
OS_Tumor43_11_236261 0.9015548 0.9293858
OS_Tumor44_12_211561 0.6793692 0.1856015
Normalbone.3. 0.1636323 0.1222070
Normalbone.UA. 0.1934500 0.1303734
Normalbone.UA2. 0.1737224 0.1604758
CRL_11372 0.9779593 0.2533844
CRL_1427 0.9598825 0.8862426
CRL_1543 0.9283976 0.7198934
CRL_2098 0.9325895 0.6360936
Ho_f.4610 0.9370865 0.6279557
HTB_85 0.9441092 0.1037401
HTB_96 0.9511175 0.9160013
ZIC4_cg12892506_3 DLX5_cg05597836_4
OS_Tumor1_08_14985_2_3 0.86033747 0.90698171
OS_Tumor2_08_226869_1 0.69614933 0.66414891
OS_Tumor3_10_201917_2_3 0.58728927 0.65086446
OS_Tumor4_00_2395 0.41747130 0.45464648
OS_Tumor5_02_2669 0.74259213 0.88244165
OS_Tumor6_02_4738 0.79690018 0.93691928
OS_Tumor7_02_4850 0.05513471 0.91753824
OS_Tumor8_02_6935 0.70742299 0.77802530
OS_Tumor9_03_1430 0.84563086 0.87202952
OS_Tumor10_03_1701 0.81318017 0.72446802
OS_Tumor11_03_220 0.72489087 0.80580733
OS_Tumor12_03_2558 0.06656780 0.14739011
OS_Tumor13_03_373 0.94011867 0.95742989
OS_Tumor14_03_82 0.52129769 0.54734874
OS_Tumor15_06_22319 0.93025191 0.94392535
OS_Tumor16_07_16581 0.67236887 0.88546907
OS_Tumor17_07_28523 0.25764851 0.45550666
OS_Tumor18_07_3212 0.33624514 0.18623351
OS_Tumor19_07_6990 0.82904776 0.88946081
OS_Tumor20_07_6990sample2 0.76836030 0.82519665
OS_Tumor21_07_7724 0.90314315 0.71810973
OS_Tumor22_08_10238 0.83715782 0.86426252
OS_Tumor23_08_14985Sample2 0.82588214 0.90655043
OS_Tumor24_08_16592 0.77582828 0.83500490
OS_Tumor25_08_21197 0.90274785 0.96119490
OS_Tumor26_08_21197Sample2 0.87385578 0.83341529
OS_Tumor27_08_222863 0.72588195 0.59849569
OS_Tumor28_08_225814 0.87931232 0.89657489
OS_Tumor29_08_226869Sample2 0.86379063 0.88825605
OS_Tumor30_08_230660 0.92495563 0.95137547
OS_Tumor31_08_4485 0.84465179 0.66948504
OS_Tumor32_09_214654 0.92036441 0.71915709
OS_Tumor33_09_214654Sample2 0.93955431 0.46490552
OS_Tumor34_10_200150 0.80337813 0.82519665
OS_Tumor35_10_201917 0.87734686 0.91265824
OS_Tumor36_10_201917Sample2 0.93627004 0.94319232
OS_Tumor37_10_202221 0.93107776 0.96075927
OS_Tumor38_10_204294 0.85892154 0.94595920
OS_Tumor39_10_204294Sample2 0.69839075 0.76396555
OS_Tumor40_10_205933 0.25850129 0.43418918
OS_Tumor41_10_225662 0.71442683 0.88154146
OS_Tumor42_10_229129 0.42328977 0.77763541
OS_Tumor43_11_236261 0.82946721 0.87242385
OS_Tumor44_12_211561 0.84785994 0.12366988
Normalbone.3. 0.06840225 0.08229536
Normalbone.UA. 0.07383344 0.12502117
Normalbone.UA2. 0.07741817 0.09563505
CRL_11372 0.05409689 0.13815797
CRL_1427 0.91730069 0.92421230
CRL_1543 0.77789918 0.94650563
CRL_2098 0.91524115 0.50725942
Ho_f.4610 0.05736418 0.51843963
HTB_85 0.95464468 0.05260867
HTB_96 0.88410529 0.92872206
#Calculate the distance
Data_dist<-dist(Data_matrix)
#Make the cluster
Data_clust<-hclust(Data_dist,method="ward")
#Save the plot as a pdf file
pdf(file="Cluster_Probes_From_genes_morethan_2_probes_and_morethan_30_methdiff.pdf")
#Plot the cluster
plot(Data_clust, cex=0.5)
dev.off() #Ends the pdf saving.
Gitte Brinch Andersen
Kandidat-Ph.d. studerende
Biomedicinsk Institut
Wilhelm Meyers Allé 4
Aarhus Universitet
DK-8000 Aarhus C
Mobil: +45 30433317
E-mail: gitt...@hum-gen.au.dk<mailto:gitt...@hum-gen.au.dk>
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