Julie -
   Since the apply functions operate on one row at a time, they
can't do what you want. I think the easiest way to solve your problem is to reshape the data set, and merge it back with the original:

dd = data.frame(Sample=c(1,1,1,2,2,2,3,3,3),
+                 Time=c(1,2,3,1,2,3,1,2,3),
+                 Mass=c(3,3.1,3.4,4,4.3,4.4,3,3.2,3.5))
rdd = reshape(dd,timevar='Time',idvar='Sample',direction='wide')
rdd$"Gain2-3" = rdd$Mass.3 - rdd$Mass.2
merge(dd,subset(rdd,select=c('Sample',"Gain2-3")))
  Sample Time Mass Gain2-3
1      1    1  3.0     0.3
2      1    2  3.1     0.3
3      1    3  3.4     0.3
4      2    1  4.0     0.1
5      2    2  4.3     0.1
6      2    3  4.4     0.1
7      3    1  3.0     0.3
8      3    2  3.2     0.3
9      3    3  3.5     0.3

You may want to avoid using special characters like dashes in variable
names.

Hope this helps.

                                        - Phil Spector
                                         Statistical Computing Facility
                                         Department of Statistics
                                         UC Berkeley
                                         spec...@stat.berkeley.edu




On Mon, 17 Sep 2012, Julie Lee-Yaw wrote:

Hi?

I have a dataframe similar to:

Sample<-c(1,1,1,2,2,2,3,3,3)

Time<-c(1,2,3,1,2,3,1,2,3)

Mass<-c(3,3.1,3.4,4,4.3,4.4,3,3.2,3.5)

mydata<-as.data.frame(cbind(Sample,Time,Mass))


? Sample Time Mass
1 ? ? ?1 ? ?1 ?3.0
2 ? ? ?1 ? ?2 ?3.1
3 ? ? ?1 ? ?3 ?3.4
4 ? ? ?2 ? ?1 ?4.0
5 ? ? ?2 ? ?2 ?4.3
6 ? ? ?2 ? ?3 ?4.4
7 ? ? ?3 ? ?1 ?3.0
8 ? ? ?3 ? ?2 ?3.2
9 ? ? ?3 ? ?3 ?3.5

where for each sample, I've measured mass at different points in time.?

I now want to calculate the difference between Mass at Time 2 and 3 for each unique 
Sample and store this as a new variable called "Gain2-3". So in my example 
three values of 0.3,0.1,0.3 would be calculated for my three unique samples and these 
values would be repeated in the table according to Sample. I am thus expecting:

mydata #after adding new variable

? Sample Time MassGain2-3
1 ? ? ?1 ? ?1 ?3.00.3
2 ? ? ?1 ? ?2 ?3.1 0.3
3 ? ? ?1 ? ?3 ?3.4 0.3
4 ? ? ?2 ? ?1 ?4.0 0.1
5 ? ? ?2 ? ?2 ?4.3 0.1
6 ? ? ?2 ? ?3 ?4.4 0.1
7 ? ? ?3 ? ?1 ?3.0 0.3
8 ? ? ?3 ? ?2 ?3.2 0.3
9 ? ? ?3 ? ?3 ?3.5 0.3

Does anyone have any suggestions as to how to do this? I've looked at the 
various apply functions but I can't seem to make anything work. I'm fairly new 
to R and would appreciate specific suggestions.?

Thanks!
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