thanks for the replies.... so, i remove the indices pv[i] and fc[i]..but when i run it like this: =============== fc=0 pv=0 for (i in 1:nrow(data)) { v1= c(y1[i,1], y1[i,2]) v2= c(y2[i,1], y2[1,2]) fc=v1-v2 w=t.test(v1,v2) pv=w$p.value }
results = cbind(row.names(y1), fc, pv) head(results) ================= i get =========== Warning message: In cbind(row.names(y1), fc, pv) : number of rows of result is not a multiple of vector length (arg 2) ============= and the "fc" values are repeated over until the end of the rows and the "pv" is the same all across the samples...how can i fix it?! ...i apologize if it's a silly situation but i'm new to this and can't get my head around it! thanks so much!! On Thu, Oct 27, 2011 at 9:02 AM, Weidong Gu <anopheles...@gmail.com> wrote: > In your loop, you assign, for example, pv twice > pv=w$p.value # pv is scalar > pv[i]= w[2] # pv is a vector > > give an example for the point > > pv=1 > pv[5]=2 > pv > [1] 1 NA NA NA 2 > > This may not be what you want. > > Weidong > > On Thu, Oct 27, 2011 at 7:40 AM, Seb <seba....@gmail.com> wrote: >> y1,y2 were designed as follow,from the original data file : >> y1=data[,1:2] >> y2=data[,3:4] >> >> ...I am a bit confused with what "redesign the whole vector again" and >> "specify indices twice " actually mean?...could u point it out in the >> script? >> >> Thanks so much I really appreciate it!! >> >> Sent from my -DROID- >> >> On Oct 26, 2011 6:56 PM, "Weidong Gu" wrote: >>> >>> If you provide an example data (y1 and y2 in the loop), you might have >>> got specific helps already. A few things in your loop seem suspicious. >>> fc and pv are vectors, and in each loop you redesigned the whole >>> vectors and specific indices twice. That may cause your problems. >>> >>> Weidong Gu >>> >>> >>> >>> On Wed, Oct 26, 2011 at 4:56 PM, Seb <seba....@gmail.com> wrote: >>> > hi all >>> > >>> > i started recently using R and i found myself stuck when i try to >>> > analyze microarray data. >>> > >>> > i use the "affy" package to obtain the intensities of the probes, i >>> > have two CTRs and two treated. >>> > >>> > HG.U133A.Experiment1.CEL HG.U133A.Experiment2.CEL >>> > HG.U133A_Control1.CEL HG.U133A_Control2.CEL >>> > 1007_s_at 2156.23115 467.75615 >>> > 364.60615 362.11865 >>> > 1053_at 88.76368 93.58436 >>> > 438.49365 357.75615 >>> > 117_at 144.00743 101.26120 >>> > 95.11117 107.01623 >>> > 121_at 551.36865 639.45615 >>> > 456.66865 435.95615 >>> > 1255_g_at 65.33164 18.39570 >>> > 14.22565 20.74632 >>> > 1294_at 106.19083 169.69369 >>> > 78.15722 81.14689 >>> > >>> > i divided the first two columns in two data.frames to divide Experim and >> CTRs >>> > >>> > then, i created a FOR loop to create a vector per each row containing >>> > a vector with two values per each gene and i wanted to do a >>> > Wilcox.test to obtain the significant genes..BUT i get a list of NULL >>> > like you can see here >>> > ..the first row works but then i get NULL down till the end of the >> array... >>> > >>> > fc pv >>> > [1,] "1007_s_at" -20.248 0.4664612 >>> > [2,] "1053_at" -344.7132 NULL >>> > [3,] "117_at" NULL NULL >>> > [4,] "121_at" NULL NULL >>> > [5,] "1255_g_at" NULL NULL >>> > [6,] "1294_at" NULL NULL >>> > >>> > the script i used is: >>> > =================== >>> > fc=0 >>> > pv=0 >>> > for (i in 1:nrow(data)) >>> > { >>> > v1= c(y1[i,1], y1[i,2]) >>> > v2= c(y2[i,1], y2[1,2]) >>> > fc=v1-v2 >>> > w=t.test(v1,v2) >>> > pv=w$p.value >>> > fc[i]= w[1] >>> > pv[i]= w[2] >>> > } >>> > >>> > results = cbind(row.names(y1), fc, pv) >>> > >>> > head(results) >>> > >>> > ================ >>> > >>> > what did i do wrong? i can't find a way around this!!! >>> > >>> > thanks so much!!! >>> > >>> > Seb >>> > >>> > ______________________________________________ >>> > R-help@r-project.org mailing list >>> > https://stat.ethz.ch/mailman/listinfo/r-help >>> > PLEASE do read the posting guide >> http://www.R-project.org/posting-guide.html >>> > and provide commented, minimal, self-contained, reproducible code. >>> > >> >> [[alternative HTML version deleted]] >> >> ______________________________________________ >> R-help@r-project.org mailing list >> https://stat.ethz.ch/mailman/listinfo/r-help >> PLEASE do read the posting guide http://www.R-project.org/posting-guide.html >> and provide commented, minimal, self-contained, reproducible code. >> > ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.