I have question about log2 transformation and performing mean on log2 data. I am doing analysis for ELISA data. the OD values and the concentration values for the standards were log2 transformed before performing the lm. the OD values for samples were log2 transformed and coefficients of lm were applied to get the log2 concentration values. I then backtransformed these log2 concentrations and the trouble started. when i take the mean of log2 concentrations the value is different than the backtransformed concentrations.
> 100+1000/2 [1] 600 > 2^((log2(100)+log2(1000))/2) [1] 316.2278 What I am doing wrong to get the different values -- View this message in context: http://r.789695.n4.nabble.com/log-transformation-and-mean-question-tp3517825p3517825.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.
