Hi All,
This is more of a general statistics question that I am not so sure about so
I need some thoughts from you experts out there. I have Chip-Seq read count
data for 2 different treatments (T1 and T2) and my final aim is to calculate
differential expression between them. I also have Chip-Seq read count data
from a "control / wild type (C)" experiment. I have 3 replicates (2
technical, 1 biological) of each of these. What is the best way to go for a
differential expression between T1 and T2? Is it best to just go straight
between T1 and T2 or is it to do a T1vsC and T2vsC and then compare the
results from these for diff expr? What do you think is better and why? I
appreciate your thoughts on this.
Sincerely,
Vishal
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