On 17 Feb 2006 at 9:51, Don Williams wrote: > Hi Rob, > > The stacking of images has been done for a > long time in laboratories using electron and > light microscopes. We did this in the late > 70s on an HP2000 system in my Institute. I > was able to entice a virologist from Holland > (despite Apartheid) to visit for a few > months. His work was mainly optical > transforms. We then developed a suite of > image processing programs using Tony > Crowther's (MRC Cambridge) ideas as a start. > But it took 8 hours or more to do a FFT, mask > and reverse FFT on a 512 x 512 matrix. I can > do it in a minute or two. And that time is > taken making the mask. The actual processing > is over before one's finger leaves the Enter Key.
Amazing, what scientists could have achieved years ago with all todays idle CPU cycles boggles the mind :-) > The colour fringes are not a problem unless > one is concerned with aesthetics. I think it > would be less work to fix the final image > rather than the individual frames. I'll look > into this. What do you use for the job? The > collector, the condenser, the objective and > the eyepiece all contribute to the problem > and a combination of optics to eliminate them > entirely would cost about £20 000 at a rough > guess. As it is the objective I used for this > lists at about £1200. Panotools could be used to batch process a set of images prior to stacking and I would guess that once the correction parameters are determined they will apply to all images captured with the same objectives and mag: http://photocreations.ca/radial_distortion/ (outline) http://www.abolais.nl/ca-cor.htm (more detailed and most suitable method for your purposes) There are less thorough options using GUI tools such as PTlens but the results would be near as good. http://epaperpress.com/ptlens/ Cheers, Rob Studdert HURSTVILLE AUSTRALIA Tel +61-2-9554-4110 UTC(GMT) +10 Hours [EMAIL PROTECTED] http://members.ozemail.com.au/~distudio/publications/ Pentax user since 1986, PDMLer since 1998
