Re: Photo micrography

[Don Williams]

Slowing is no good, they have to be 
absolutely still -- killed and fixed. 
It's impossible to take a stack of 
pictures of live animals small or large. 
Even taking a dozen pictures of a plant 
is impossible unless it's rigid or 
rather substantial -- like a cactus or a 
fungal fruiting body (mushroom). A dead 
insect works as long as you're quick 
about it. On the other hand you can do 
stereo pictures of living things (flash 
helps). But viewing them is something 
that some people simply cannot do -- 
without optical help such as a viewer or 
glasses (red/green, Polaroid or prism).

When a series of pictures is taken in a 
compound microscope the successive 
images may be displaced vertically (y) 
or horizontally (x) by small amounts as 
you move down or up (z); magnification 
also changes. There are stacking 
programs such as Helicon Focus, 
AstroStack and CombZ that have 
corrections -- either automatic or 
manual to fix these problems. Some do it 
well, others don't. But there's a hell 
of a lot more to this. I have two; 
Helicon Focus and CombZ. For me CombZ 
works best.

It would help to contact me off list. I 
really don't have time to wade through 
all the posts these days

Don
mike wilson wrote:
From: "Leon Altoff" <[EMAIL PROTECTED]>
Date: 2005/04/28 Thu PM 01:33:21 GMT
To: "pentax-discuss@pdml.net" <pentax-discuss@pdml.net>
Subject: Re: Photo micrography
On Thu, 28 Apr 2005 10:52:54 +0300, Don Williams wrote:

Hi Leon,
Depth of field is not something you can 
get very easily. If you have subjects 
with real depth the answer is to use 
Helicon Focus or ComZ to stack images. 
They have a system like this at the museum where I do volunteer work. 
I have plans of playing with it.  The problem is that I am taking
pictures of live marine animals in the range of 0.5 - 2 mm and they
don't sit still while you take a hundred photos of them (I'm lucky if
hey stay in the frame while I press the shutter release).  They are
also very 3 dimensional.

If you have any ideas I'd be happy to hear them.

There are various techniques for slowing them down by varying the environmental 
vairiables.
mike
-----------------------------------------
Email sent from www.ntlworld.com
virus-checked using McAfee(R) Software
visit www.ntlworld.com/security for more information