What is your alternative?  Preserve in the analysis the arbitrary positioning 
of specimens in a photo or scan? Procrustes does have biases but it would have 
to be replaced with some other procedure. Perhaps one has some known reference 
points but then variation in those points will become very important. 
Jim__________________F. James Rohlf, Distinguished Prof. Emeritus Dept. 
Anthropology and Ecology & Evolution Stonybrook University
-------- Original message --------From: [email protected] Date: 9/9/25  
2:49 AM  (GMT-05:00) To: Jacqueline Silviria <[email protected]> Cc: 
[email protected] Subject: Re: [MORPHMET2] Effect of Procrustes 
alignment on parameter estimates Dear Jacqueline Yes it could be for instance 
for ancestral state reconstruction. What I am thinking about is whether anybody 
has done a simulation study where they simulate shape data, procrustes align 
the data, infer some quantity of interest (could be ancestral state, or 
something else) and then look at how the estimated quantity compares to the 
true quantity (the one used for simulation) with or without procrustes 
alignment. I would expect procrustes alignment to have an effect as it is not 
really a part of any of the stochastic models I am aware of. I am sorry if my 
question is very odd, I do not have a strong background in geometric 
morphometrics so I might have misunderstood something. Thank you!Best 
regards,Sofia On 9 Sep 2025, at 03.45, Jacqueline Silviria 
<[email protected]> wrote:Just to be clear, by parameter estimation for 
stochastic models, you mean for the purpose of ancestral state 
reconstruction?Jacqueline S. Silviriashe/her/hersThe Last King of the 
JungleDepartment of Earth & Space ScienceUniversity of WashingtonSeattle, WA, 
[email protected][email protected] profileSent from my 
iPhoneOn Sep 8, 2025, at 6:02 AM, Sofia Stroustrup <[email protected]> 
wrote:Dear all, I am wondering whether any of you are aware of a study that 
investigates the effect of Procrustes alignment on parameter estimation for 
instance under a Brownian motion or Ornstein-Uhlenbeck process? What I am 
thinking about is, if we simulate data under a stochastic model, procrustes 
align the data and then do parameter estimation how does Procrustes alignment 
affect the estimates. I have not been able to find any study investigating 
this. Thank you very much for your help! Best regards, Sofia (PhD student at 
University of Copenhagen)

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