Hello everyone, I work with fluctuating asymmetry of wings and I am currently using individual values generated by a Procrustes ANOVA to perform an allometry regression and a principal component analysis of asymmetric shape variation. I have run the same analyses with datasets of other traits with matching symmetry and everything went smoothly. However, in my wing dataset, MorphoJ does not allow me to select a fundamental classifier for my analyses, which corresponds to the environmental condition (impacted site, reference site).
Whenever I try to perform a PCA considering the differences of environmental conditions of each individual, the analysis appears on my project tree, but with no results. I can perform it without pooling within the environmental condition classifier and see the results. Unfortunately, a PCA without identifying which condition each individual belongs to would not be useful in any way to respond the questions of my research. I tried to insert the environmental condition effect in the Procrustes ANOVA and this message appeared on the reports tab: *"Procrustes ANOVA: The effect indicated for Error 2 has no degrees of freedom. The effect was dropped from the analysis." . *I found a discussion <https://www.mail-archive.com/[email protected]/msg02885.html> on Morphmet about this, but I'm not quite sure as to what to do with this information. If the issue is related to the error 2 (landmark error), why do I only get this warning if I try to include an additional effect on the analysis? What can I do to obtain landmark errors with degrees of freedom over zero and perform this PCA successfully? This only happened for analyses of this trait, which has an appropriate amount of landmarks according to the sampling size. I have tried to run the same analyses with a new set of landmarks and nothing has changed. Is anyone familiar with this issue? Let me know if any more information is needed! Best regards, Helena. -- You received this message because you are subscribed to the Google Groups "Morphmet" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To view this discussion on the web visit https://groups.google.com/d/msgid/morphmet2/8685852c-47be-419c-baf0-6f385d6fe0ccn%40googlegroups.com.
