Dear Takehide Thank you for the tip, I could find dam dcm strains, but I am really looking for one that is also endA so that plasmid quality and yield does not suffer.
all the best, bjorn ______O_________oO________oO______o_______oO________O_________oO________ Björn Johansson, Assistant Professor Department of Biology University of Minho Campus de Gualtar 4710-057 Braga PORTUGAL https://metabolicengineeringgroupcbma.github.io | www.bio.uminho.pt Office direct +351-253 601517 | mob. +351-967 147 704 | Dept secretary +351-253 60 4310 On Thu, Dec 6, 2018 at 11:33 PM M. Take at Tsukuba Science City < [email protected]> wrote: > Dear Bjorn , > > Can you find such the strain in the National BioResource Project stock? > https://shigen.nig.ac.jp/ecoli/strain/ > > Takehide > NBRP E.coli Strain - shigen.nig.ac.jp > <https://shigen.nig.ac.jp/ecoli/strain/> > "jw621-am"といった単語を二重引用符 (") で囲むと、完全一致検索を行うことができます。 > shigen.nig.ac.jp > > ------------------------------ > *差出人:* [email protected] < > [email protected]> が Bjorn <[email protected]> の代理で送信 > *送信日時:* 2018年12月7日 0:23 > *宛先:* [email protected] > *件名:* Request for dam–/dcm– E. coli > > Hi, I wonder if anyone would be willing to share an > dam–/dcm– negative E. coli like for example the NEB dam–/dcm–. > > I have a plasmid I need to produce w/o methylation. > > Thanks! > /Bjorn > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
