On a completely different note- DK, Wo, It's cool to see your name pop up in my mail box. I've missed this community, I guess there haven't been too many mol bio questions lately. So, it's cool to see you guys back! :)
On 9 November 2017 at 19:21, DK <[email protected]> wrote: > In article <[email protected]>, Megha > Goyal <[email protected]> wrote: > >Dear All, > > > >We are doing fermentation for recombinant protein using E.coli culture. > >Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after > >IPTG Induction). But recently we observed that the cell pellet harvested > >are of slimy consistency and they do not homogenise in the washing buffer > >(tris-nacl-edta). We observe thread like consistency and no matter of > >stirring it do we get a homogeneous suspension. > > > >Can someone guide me what is the reason for such phenomenon. > > Partial cell lysis. "Slime" = chromosomal DNA. > > DK > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
