I am more than sure the culture is contaminated. Raise mother culture from single well isolated clone.
On Nov 9, 2017 10:35 PM, <[email protected]> wrote: > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. Slimy recombinant e.coli pellet (Megha Goyal) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 9 Nov 2017 11:41:15 +0530 > From: Megha Goyal <[email protected]> > Subject: Slimy recombinant e.coli pellet > To: [email protected] > Message-ID: > <CAEhjShWGL0jQ+Gaz7XAv_dczNxkzAazsKN06Fzc=Ji7Q5mEWOg@ > mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Dear All, > > We are doing fermentation for recombinant protein using E.coli culture. > Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after > IPTG Induction). But recently we observed that the cell pellet harvested > are of slimy consistency and they do not homogenise in the washing buffer > (tris-nacl-edta). We observe thread like consistency and no matter of > stirring it do we get a homogeneous suspension. > > Can someone guide me what is the reason for such phenomenon. > > Thanks > > megha > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 137, Issue 1 > *************************************** > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
