Dear Tieqiao,
what conditions have you already tested? You may try different salts,
salt concentrations, pH and possibly addig (preferably non-ionic)
detergents. In worst case, go denaturing (Urea, chaotropic salts, MgCl2).
Have fun!
Wo
On 2017年05月19日 13:40, TieQiao Wu wrote:
Dear all:
Does anyone have good method to prevent protein from aggregation during protein
purification from E. coli and thrombin cleavage?
Thanks a lot!
Tieqiao
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