Hi, I’ve never made it, but found a collection of hints on Research Gate. Maybe the answer is here? (Scroll down to Claudia Schinke’s answer, that seems to be the most complete).
https://www.researchgate.net/post/Does_anyone_understand_how_to_make_skim_agar_media Barbara On 13 May 2016, at 13:03, [email protected]<mailto:[email protected]> wrote: Send Methods mailing list submissions to [email protected]<mailto:[email protected]> To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/methods or, via email, send a message with subject or body 'help' to [email protected] You can reach the person managing the list at [email protected] When replying, please edit your Subject line so it is more specific than "Re: Contents of Methods digest..." Today's Topics: 1. Query about skim milk agar ([email protected]) From: <[email protected]> Subject: Query about skim milk agar Date: 12 May 2016 16:27:00 GMT-4 To: <[email protected]> On Friday, March 14, 1997 at 1:00:00 PM UTC+5, Kimberly Walker wrote: How in the world do you make this stuff? I tried to filter sterilize the skim milk, but it wouldn't go through the filter. Autoclaved milk carmelizes. Does anyone have expeience with this? I am also making skim milk agar with 1% tryptone... before autoclaving it is prooperly dissolved and is clear solution...but after autoclaving there are clots of milk in media and the solution is not clear and properly dissolved, although it solidifies...Kindly guide where i am doing the wrong? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods --------------------------------------------- Barbara MacGregor University of North Carolina Department of Marine Sciences 3117C Venable Hall (a/k/a Murray) CB#3300 Chapel Hill NC 27599 Email [email protected]<mailto:[email protected]> Cell +1 (919) 260-6038 Fax +1 (919) 962-1254 Lab 3110 Venable Hall
_______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
