one of the best options to remove traces of nucleic acids is to rinse the glassware after thorough wash with 1M NaOH. cheers!
On Mon, Oct 19, 2015 at 12:53 PM, John Dudgeon <[email protected]> wrote: > Does anyone have an opinion on the utility of dilute HNO3 (~2-3%) for > removing residual DNA and proteins from glassware and stainless steel lab > tools. Our water is very hard here (>35 grains hardness), so we use HNO3 to > periodically clean lab glassware from hard water deposits in large dunk > tanks. > > Our experience in our tace metals and DNA lab suggest nitric acid is far > less damaging to polyethylene and polypropylene plastics, glassware with > painted volumetric markings and stainless steel tools than dilute NaClO > (chlorine bleach) solutions. We have used HNO3 for years to digest tissues > and metalloproteins prior to ICP-MS analysis where we are trying to release > incorporated trace metals, and I wonder if it might be equally as effective > as NaClO for removing DNA and RNA on labware used in forensic DNA and/or > protein applications. There are many papers that discuss the method of > action of HNO3 on organic molecules, but I haven't seen anybody > specifically referencing its effect on DNA/RNA. > > Any opinions? > > John > > -- > John Dudgeon, Ph.D. > Associate Professor, Department of Anthropology > Director, Center for Archaeology, Materials and Applied Spectroscopy > (CAMAS) > > ------------------------------------------------------------------------------------------------ > > Idaho State University, 921 S. 8th Avenue, Stop 8005, Pocatello, ID > 83209-8005 > Phone: (208) 282-3862 - FAX: (208) 282-4944 > http://anthropology.isu.edu/dudgeon.shtml > http://www.isu.edu/camas/ > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > -- *CONFIDENTIAL COMMUNICATION* *E-mails from The Open University of Sri Lanka normally contain confidential and privileged material, and are for the sole use of the intended recipient. Use or distribution by an unintended recipient is prohibited, and may be a violation of law. If you believe that you received this e-mail in error, please do not read this e-mail or any attached items. Please delete the e-mail and all attachments, including any copies thereof, and inform the sender that you have deleted the e-mail, all attachments and any copies thereof. Thank you.* _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
