Hello, I have used both the pLys and standard BL21(DE3). I didn't find a difference in the expression levels. Some people might have more experience depends on applications or protein etc. Here is the function of pLys in the strains of coli (from Internet):
"The pLysS plasmid carried by the BL21 Star(tm)(DE3)pLysS strain produces T7 lysozyme to reduce basal level expression of the gene of interest. pLysS confers resistance to chloramphenicol (CamR) and contains the p15A origin. This origin allows pLysS to be compatible with pUC- or pBR322-derived plasmids." So those cells can tolerate another antibiotic (chloram.) and the pLysS reduces basal level expreesion of the gene of interest. Toufic El Arnaout On Fri, Mar 28, 2014 at 5:03 PM, <[email protected]>wrote: > Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. RE: E coli BL21?DE3 ? (Alejandro Miguel Martin Dunn) > 2. RE: E coli BL21?DE3 ? (DK) > 3. Re: Publishing Equipment Twaks? (Dr Engelbert Buxbaum) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 27 Mar 2014 08:10:46 -0400 > From: Alejandro Miguel Martin Dunn <[email protected]> > Subject: RE: E coli BL21?DE3 ? > To: "[email protected]" <[email protected]> > Message-ID: > <[email protected]> > Content-Type: text/plain; charset="iso-8859-7" > > It's probably 'lambda' in capital letters (quite similar to capital > delta). DE3 is a lambda prophage, hence the name. > > Hope it helps, > alejandro > > -----Original Message----- > From: [email protected] [mailto: > [email protected]] On Behalf Of WS > Sent: Thursday, March 27, 2014 2:11 AM > To: [email protected] > Subject: E coli BL21?DE3 ? > > Dear colleagues, > > While in search for BL21(DE3) & friends, I discovered only some vials > labelled "BL21ÄDE3" and some labelled "BL21ÄDE3pLYSE". I suppose that's > just a mis-spelling and thus exactly what I'm looking for, as google yields > exactly 1 hit for "BL21ÄDE3" and Pubmed doesn't return any, also when I'm > checking for"BL21Ä(DE3)". And such a denomination doesn't make any sense > to me, too, as DE3 is not part of the natural genotype. > > I'd appreciate any comments. > > Thanks! > > Wo > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > > > > ------------------------------ > > Message: 2 > Date: Fri, 28 Mar 2014 02:43:41 GMT > From: [email protected] (DK) > Subject: RE: E coli BL21?DE3 ? > To: [email protected] > Message-ID: <[email protected]> > > In article <[email protected]>, Alejandro > Miguel Martin Dunn <[email protected]> wrote: > >It's probably 'lambda' in capital letters (quite similar to capital > delta). DE3 > > is a lambda prophage, hence the name. > > My thought exactly. Particularly when combined with DE3pLysE, which is > a certain to be (DE3) strain carrying pLys plasmid. > > DK > > > >Hope it helps, > >alejandro > > > >-----Original Message----- > >From: [email protected] > > [mailto:[email protected]] On Behalf Of WS > >Sent: Thursday, March 27, 2014 2:11 AM > >To: [email protected] > >Subject: E coli BL21?DE3 ? > > > >Dear colleagues, > > > >While in search for BL21(DE3) & friends, I discovered only some vials > labelled > > "BL21ÄDE3" and some labelled "BL21ÄDE3pLYSE". I suppose that's just a > > mis-spelling and thus exactly what I'm looking for, as google yields > exactly 1 > > hit for "BL21ÄDE3" and Pubmed doesn't return any, also when I'm checking > > for"BL21Ä(DE3)". And such a denomination doesn't make any sense to me, > too, > > as DE3 is not part of the natural genotype. > > > >I'd appreciate any comments. > > > >Thanks! > > > >Wo > >_______________________________________________ > >Methods mailing list > >[email protected] > >http://www.bio.net/biomail/listinfo/methods > > > > > > > ------------------------------ > > Message: 3 > Date: Fri, 28 Mar 2014 16:36:12 +0100 > From: Dr Engelbert Buxbaum <[email protected]> > Subject: Re: Publishing Equipment Twaks? > To: [email protected] > Message-ID: <[email protected]> > Content-Type: text/plain; charset="us-ascii" > > In article <[email protected]>, > [email protected] says... > > > > Dear Colleagues, > > > > from time to time I'm doctoring some lab equipment to make it better > suit my needs. Is there any journal that would accept these tweaks in > exchange for a few impact points? > > > BioTechniques might be worth a try, or perhaps a short paper in Anal. > Biochem. if you can show that your tweak is more generally useful. > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 105, Issue 11 > **************************************** > > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
