On Wednesday, June 15, 1994 8:17:40 PM UTC-4, "Glenn Cantor" wrote: > I have transfected cells with a plasmid containing a hygromycin > selectable marker. To compare transfection efficiency in various > cell lines, I would like to compare the amounts of hygromycin > phosphotransferase which the lines express. Does anyone know if > antibodies or an antibody-based kit are available for detection of > hygromycin phosphotransferase? (5'-3' has an ELISA for detection of > neomycin phosphotransferase II, but doesn't have one for hygromycin.) > > Thanks! > > Debra Hoover
Can someone provide a reference regarding spontaneous mutation of CHO-K1 (untransfected) to become hygromycin B resistant? Is there data on the IC50 of hygromycin B on untransfected CHO-K1? _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
