In article <c417e4f4-0961-4518-983e- [email protected]>, [email protected] says... > > Hi all, I have a PCR problem and I can really use some help. This is an > established protocol we've been using for genotyping for a while. I'd say > half of the time it works beautifully. Sometimes we get multiple bands. > Sometimes I get smears for one set of primers but not the other set. Once I > got nice bands for some DNA sample but smear for others while I extracted > those samples together. I use the DNA sample for other PCRs and they work > just fine. I use my mastermix and PCR machine for other PCRs too so I don't think there's a problem there. Can anybody give me some pointers as to why this keeps happening? > > Thank you very much!
Could it be that some of your samples contain compounds that inhibit the polymerase? -- Car: Erratically moving obstacle on the road _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
